The expression analysis facility has a Nanodrop Spectrophotometer
available for use during normal working hours.
This device will perform full-spectrum UV-Vis absorbance analyses
(200-800 nm), and is designated for use in the facility for measuring
absorbance of DNA, RNA, microarray labeling dyes, and proteins
(while the nanodrop can read cell culture ODs and protein assays
utilizing binding days, such as the Bradford assay, our instrument
is not to be used for those types of readings). Advantages of the
nanodrop include:
* 1 ul only of sample required
* Large dynamic range: 2-3700 ng/ul of ds DNA (manufacturer’s
specs, this is probably pushing it at both ends)
* No dilutions for most samples
* No cuvettes or capillaries
* 10 second measurement
Nanodrop spectrophotometer can be used for fast analysis
of very low-volume samples. This device will perform
full-spectrum UV-Vis absorbance analyses (200-800 nm),
and ais designated for use in the facility for measuring
absorbance of DNA, RNA.
|
| Optical Specifications |
Description
|
| Wavelength range |
200-800 nm (190-1100 nm) |
| Straylight |
< 0.03 % |
| Resolution |
< +1.0 nm |
| Wavelength accuracy |
< ± 0.1 nm |
| Wavelength reproducibility |
< ±0.02 nm for 10 consecutive scans (NIST 2034) |
| Photometric accuracy |
< ±0.01 A at 235.0 257.0 313.0 nm |
| Photometric noise |
< 0.01 A 30 scan at 0 A , 500 nm |
| Photometric stability |
< 0.01 A/min at 0 A , 500 nm |
| Baseline flatness |
< 0.01 A 0.5 Second blank ,0.5 second scan at 0 A , 500 nm |
| Scan time |
2-1000 ms |
| Typical scan time |
0.1 s |
| Light source |
Deuterium+Tungsten lamp |
| Detector |
2000 Multichannel |
| Sample Specifications |
|
| sample volume |
700-4000 nL or 3000-4000 nL |
| Light path |
0.2 mm or 1 mm |
| Physical Specifications |
Description
|
| Communication |
USB 2 |
| Software |
Windows Xp ,7,8 |
| Height * Width * depth |
13 * 33 * 50 cm |
| Weight |
10 kg |
| Power |
220 V AC 50/60 Hz |
|